Consequences on Gametogenesis and Reproduction Performances of a High Carbohydrate Nutrition During the Whole Reproductive Cycle of Male and Female Trout
Bibliographic record
Abstract
Salmonids and more particularly rainbow trout are carnivorous species, and thus usually consider as glucose‐intolerant (GI) species and poor user of dietary carbohydrates displaying a decrease in growth and a persistent postprandial hyperglycaemia when fish meal is substituted at more than 20% by digestible carbohydrates in the diet. Nevertheless, these findings were mainly deduced from experiments conducted on immature juvenile trout and only scarce data are available in sexually mature males and females. In this regard, we investigated the impact of a high carbohydrate diet (HC diet) on gametogenesis and reproductive performances of 2 year‐old broodstocks fed with such a diet a whole year. Our results showed that both males and females ate and growth well when fed the HC diet even if, at the end of the trial, males, but not females, displayed growth retardation compared to control males. The glycaemia of fish fed the HC diet was higher than fish fed the control diet independently of the sex but these fish were not hyperglycaemic. Surprisingly, both sex displayed a precocious maturation. Whole body and gonads biochemical composition of fish did not differ during the whole year from one treatment to the other whereas liver of fish fed the HC diet retained more glycogen but less proteins compared to the control fish. We also monitored glucose metabolism modifications at the molecular level in both livers and gonads at different gametogenesis time points. Our results demonstrated that glucose metabolism strongly varied in female liver during gametogenesis independently of the diet whereas in male no significant difference where found. By contrast in both ovaries and testis, glucose metabolism related genes were found differentially expressed during gametogenesis but independently again of the diet. At spawning period, we performed cross‐fertilization between groups. For the 4 groups, both fertilization and early developmental rate were equivalent from one group to the other. In overall, our results highlight strong but sex‐dependent effects of a high carbohydrate diet on both oogenesis and spermatogenesis and a variation of glucose metabolism in both liver and gonads. However, these effects do not seem to affect reproductive performances. Support or Funding Information This study was supported by the French National Research Agency (ANR‐18‐CE20‐0012‐01 ‘SweetSex’). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".