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Functional Implications of Sexual Dimorphism of Transporter Patterns along the Rat Nephron

2019· article· en· W3174382482 on OpenAlexaffabout
Rui Hu, Alicia A. McDonough, Anita T. Layton

Bibliographic record

VenueThe FASEB Journal · 2019
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicIon Transport and Channel Regulation
Canadian institutionsUniversity of Waterloo
FundersNational Institutes of Health
KeywordsNephronReabsorptionCotransporterInternal medicineEndocrinologySexual dimorphismTransporterChemistryKidneyApical membraneRenal physiologyTubuleWater transportBiologySodiumMedicineMembraneBiochemistryWater flow

Abstract

fetched live from OpenAlex

The goal of this study is to investigate the functional implications of the sexual dimorphism in transporter patterns along the rodent nephron as reported by Veiras et al. (J Am Soc Nephrol 2018). To do so, we developed sex‐specific computational models of water and solute transport from Bowman space to the papillary tip of a superficial nephron of the kidneys of the male and female rats. The models account for the sex differences in expression levels of the apical and basolateral transporters, in single‐nephron glomerular filtration rate, and in tubular dimensions. Model simulations predict that ~70 and 40% of the filtered volume is reabsorbed by the proximal tubule of the male and female rat kidneys, respectively. The lower fractional volume reabsorption in the female rat can be attributed to their smaller transport area and lower aquaporin‐1 expression level. The latter also results in a larger contribution of the paracellular pathway to water transport. Similar fractions of the filtered Na + are reabsorbed by the male and female proximal tubule models, respectively. The lower fractional Na + reabsorption in female is due primarily to their smaller transport area, lower Na + /H + ‐exchanger (NHE3) activity, and lower claudin‐2 abundance. This results in significantly larger delivery of water and Na + to the downstream nephron segments in female. Unlike in the proximal tubule, the distal nephron of the female rat exhibits higher expression levels in key Na + transporters, including Na + ‐K + ‐Cl − cotransporter, Na + ‐Cl − cotransporter, and epithelial Na + channel. Not only do the higher expression levels of these transporters account for the enhanced water and Na + transport along the female distal nephron, relative to male, but they may also contribute to the female rat's ability to excrete a saline load more rapidly than male. Support or Funding Information This research was supported by the Canada 150 Research Chair program and by the National Institutes of Health: National Institute of Diabetes and Digestive and Kidney Diseases, grant R01DK106102. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .

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How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.495
Threshold uncertainty score0.295

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.216
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2019
Admission routes2
Has abstractyes

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