Radial Glia and Cell Heterogeneity Across The Lizard Central Nervous System: Distal Spinal Cord Rupture Does Not Induce a System‐Wide Response
Bibliographic record
Abstract
Radial glia are resident neural stem/progenitor cells of the central nervous system of many non‐mammalian species. Radial glia are capable of spontaneously generating new neurons, and are considered to be crucial for both brain development and injury‐mediated regeneration. Recently, radial glia have also been identified in the tail spinal cord of the lizard Eublepharis macularius (the leopard gecko), a species capable of tail spinal cord regeneration. Here, we investigated whether similar populations of radial glia were present within the body spinal cord (bSC) and brain. Using a bromodeoxyuridine pulse‐chase strategy, we determined that label‐retaining cells were found throughout the bSC and the forebrain following a 20‐week chase. Next, we established that populations of cells lining the central canal (of the bSC) and ventricular system (of the brain) express a panel of radial glia markers, including the transcription factor SOX2 and the intermediate filaments GFAP and Vimentin. However, Vimentin expression was conspicuously absent from the diencephalon (except for the hypothalamic eminence), as well as the midbrain and hindbrain. Further, cell populations lining the central canal of the bSC include both radial glia and cells with a neuronal phenotype (as evidenced by expression of the neuronal marker HuCD). An identical diversity of cell types is also reported for in the regeneration‐competent tail spinal cord. Finally, we determined that constitutive proliferation by cells lining the central canal (of the bSC) and the ventricular system (of the forebrain) are essentially unaltered in response to distal central nervous system injury (tail spinal cord rupture as a result of tail loss). Combined, our data demonstrates that radial glia are present throughout the central nervous system of the gecko, that the bSC is essentially identical to that of the tail, and that the bSC and forebrain are resilient to distal spinal cord injuries. Support or Funding Information Natural Sciences and Engineering Research Council Discovery Grant 400358 (MKV), Canadian Foundation for Innovation Leaders Opportunity Fund 25371(MKV) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".