Artificially Sweetened Vitamin Drink Consumption Reduces Insulin Sensitivity and Alters One‐Carbon, B‐Vitamin Dependent Metabolism in Adolescents
Bibliographic record
Abstract
Importance Discretionary fortified beverages contain several ingredients such as vitamins and minerals in excess amounts, yet their consumption and sales have been burgeoning. Marketed as providing a unique health benefits to consumers, concerns have been raised about the overconsumption of some nutrients, especially in children and adolescents. Objective To evaluate the impact of discretionary fortified beverage consumption on energy metabolism, gut hormones and metabolic profiles of adolescents. Design A double blind, randomized, placebo‐controlled crossover trial examined the metabolic effects of a concentrated, small format B‐vitamin fortified beverage. Healthy adolescents (10 males, 10 females, 13–19y) completed two trials separated by at least 1 week, consuming either water as placebo (PL) or an artificially sweetened discretionary fortified beverage (FB, 1.5ml/kg), which were given 40min prior to an oral glucose tolerance test (OGTT). Method Blood samples were collected at baseline and at different time‐points throughout the OGTT for insulin, gut hormones and 1 H‐NMR spectroscopy‐based metabolomics analyses. To evaluate gene‐metabolic interactions, a group of the most common single nucleotide polymorphisms (SNPs) linked to B‐vitamin metabolism was also assessed. Results Plasma glucose was not different between the treatment groups (p=0.111). However, plasma insulin was significantly higher in the FB group (p=0.003) and accompanied by a 28% decrease in insulin sensitivity in FB compared to PL as indicated by the composite Insulin Sensitivity Index (ISI). Gut hormone secretion was also differentially modulated by the consumption of FB. We found a significant increase in AUC of GLP‐1 (p<0.001), glucagon (p=0.015), PYY (p=0.001) and C‐peptide (p=0.017) in FB compared to the PL group. Employing 1 H‐NMR metabolomics, we also show perturbations to one carbon, B‐vitamin linked sulfur amino acid metabolism with FB consumption. This metabolic response was dependent on genotypic variance at rs651852 (Betaine Homocysteine methyltransferase, BHMT, p<0.05). Conclusion Current data provide evidence that FB are not inert, but may promote insulin resistance, perturb one carbon, B‐vitamin‐linked metabolism and differentially modulate gut hormone secretion in adolescent consumers. Results may warrant reconsideration of regular, artificially sweetened FB consumption for adolescents at risk for obesity and metabolic disease. Support or Funding Information The research was funded by the Alberta Children's Hospital Research Institute and the National Science and Engineering Council of Canada (JS). SM is funded through an Eyes High Postdoctoral Fellowship and an Alberta Innovates Health Solutions Postdoctoral Fellowship. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".