Functional Expression of MRP4 in the Ciliary Epithelium of the Eye
Bibliographic record
Abstract
The multidrug‐resistance associated protein 4 (MRP4) is an efflux pump that transports organic anions, including para‐ aminohippurate. Transepithelial transport of para ‐aminohippurate by the ciliary epithelium was previously demonstrated (Pelis and Delamere, The FASEB Journal 23, Abstract #797.9, 2009). This led to the hypothesis that MRP4 contributes to organic anion transport by the ciliary epithelium. MRP4 expression was examined using reverse transcriptase PCR, Western blotting and functional assays. MRP4 mRNA and protein were detected in the freshly dissected human ciliary body and in a simian virus transformed cell line derived from human ciliary body epithelial cells (SVHCE cells). MRP4 protein levels (relative to total protein) in the native tissue and cultured cells were comparable. In SVHCE cells, the intracellular accumulation of the fluorescent MRP4 substrate bimane glutathione increased in a dose‐dependent manner in the presence of the MRP4 inhibitors, probenecid and indomethacin. These data indicate that MRP4 contributes to organic anion transport by the human ciliary epithelium. The functional expression of MRP4 in SVHCE cells makes them a potentially useful system for examining MRP4‐mediated organic anion transport by ciliary epithelia. Supported by NIH.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".