Cloning and characterization of novel CTP: phosphoethanolamine cytidylyltransferase (Pcyt2) isoforms made by alternative splicing
Bibliographic record
Abstract
Pcyt2 controls the rate limiting step of de novo biosynthesis of phosphatidylethanolamine. We previously identified the exon‐7 skipping as a mechanism for alternative splicing of two different Pcyt2 isoforms, Pcyt2α and Pcyt2β. Homology searches of genome databases indicated the presence of additional Pcyt2 transcripts. In order to isolate and characterize those alternative transcripts, we used total cDNA prepared from C 2 C 12 and multiple mouse tissues. Methodology included specific PCR amplification, cloning into pGEMt vector and sequencing. We detected two distinctive Pcyt2 isoforms, named γ1 and γ2. The Pcyt2γ1 is a longer form, composed of exons 1–8 and two additional regions from introns 7 and 8. The first intronic segment of 184 bp located at the beginning of intron 7 was named γ‐specific region 1. Next, exon 8 was followed by 258 bp of sequence from intron 8, named γ‐specific region 2. The new isoforms resemble the first half of Pcyt2α in containing the first catalytic site and in maintaining the exon 7 linker peptide region. Pcyt2γ1 contains two additional regions made of partial introns 7 and 8, while Pcyt2γ2 retains only parts of intron 8. This composition made the C‐terminal ends of both γ1 and γ2 completely different from isoform Pcyt2α. Both isororms were expressed in different mouse tissues as well as in mouse embryos at different stages of development. This research was supported by CIHR research grant.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".