Cell physiology of fish gill mitochondrion rich cells
Bibliographic record
Abstract
Regulation of both acid‐base and ion levels are essential for fish survival. Specialized gill mitochondrion‐rich (MR) cells are responsible for the active transport required to maintain homeostasis. We have utilized an intracellular pH (pHi) imaging system to uncover the specific cellular mechanisms responsible for ion‐transport in gill MR cells. We provide important new information on the use of apical Na+ channels coupled with basolateral electrogenic Na+/HCO3‐ co‐transporters acting in export mode to accomplish transepithelial Na+ uptake. In addition, we present evidence for Cl‐/HCO3‐ exchangers (CBE) acting apically for transepithelial Cl‐ uptake on one MR cell population and a second CBE located basolaterally for pHi regulation. We also present evidence for basolateral Cl‐ dependent Na+/H+ exchangers playing a role in pHi regulation. Our data on dopamine and PMA stimulated Na+‐dependent transport processes suggest are the first evidence for direct stimulation of ion transport in MR cells. Our results have culminated in new models for both acid and base secreting cells and their connection to overall Na+ and Cl‐ uptake respectively. Funded by a NSERC Discovery Grant to GGG.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".