Extremely alkaline intracellular pH in the anterior stomach of larval yellow fever mosquitoes ( <i>Aedes aegypti</i> )
Bibliographic record
Abstract
The anterior stomach of larval Aedes aegypti is an alkalinizing organ where basolateral V‐ATPase drives luminal alkalinization to a pH of up to 12. However, the apical mechanisms involved are unknown. We hypothesized that luminal alkalinization involves absorption of H + via apical H + channels. Such a system would require increased intracellular pH (pH i ) to function effectively. pH i was monitored using live cell imaging and BCECF as a pH‐dependent fluorophore. Serotonin (0.2 ìM), which stimulates luminal alkalinization, increased pH i in the anterior stomach from 7.0 ± 0.1 to 7.7 ± 0.1 (N = 64), whereas pH i was not affected in the posterior stomach (6.7 ± 0.1 before and 6.8 ± 0.1 after serotonin; N = 19). Perfusing the lumen with pH 10 saline increased pH i to 8.6 ± 0.1 (N = 41), suggesting a marked apical H + permeability. However, in presence of luminal Zn 2+ (10 ìM) similarly high pH i values were recorded (8.2 ± 0.1; N = 15), indicating that an apical proton channel, if present at all, cannot be blocked with Zn 2+ . The rate of pH change after switching to pH 10 in the lumen in the presence or absence of luminal Zn 2+ was also not different. Together these results show that remarkably alkaline pH i values can be observed in the anterior stomach of larval Aedes aegypti . However, further investigation is required to determine the transporters involved in this unique physiological system. This work was supported by NIH (1R01AI063463‐01A2).
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".