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Genetic influence on fatty acid composition of human breastmilk

2012· article· en· W3177144424 on OpenAlexaboutno aff
Carol L. Cheatham

Bibliographic record

VenueThe FASEB Journal · 2012
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMetabolomics and Mass Spectrometry Studies
Canadian institutionsnot available
Fundersnot available
KeywordsArachidonic acidDocosahexaenoic acidDocosapentaenoic acidBreastfeedingFatty acidFADS2Breast milkGenotypeLactationFood scienceChemistryBiologyBiochemistryPolyunsaturated fatty acidMedicineGeneticsGenePregnancyPediatrics

Abstract

fetched live from OpenAlex

Objective The cognitive advantage conferred by breastfeeding may be dependent on maternal FADS2 genotype. In a Canadian sample (Xie & Innis, 2008), fatty acid content of human milk related to genotype at rs174575 on the FADS2 gene. This study was undertaken to explore this in a sample from the USA. Methods Breastfeeding dyads were enrolled when infant was 3–4 months of age (n=200). Mothers collected a morning sample by emptying one entire breast, agitating, aliquoting, and transporting to the lab on ice. Samples were stored at −20 F until lipids were extracted and saponified. Fatty acids were trans‐methylated to methyl esters that were analyzed using capillary gas chromatography. DNA was extracted from saliva using a QIAcube robotic station (Qiagen), as per manufacturer's protocol. 20 ng DNA was used with validated primers from Applied Biosystems. The real time PCR assay was run on an Eppendorf thermal cycler. Results Maternal genotype is related to fatty acids: homozygous recessive (GG) had lower fatty acid levels than wildtype (CC). Levels of arachidonic acid, docosapentaenoic acid (n‐3), and docosahexaenoic acid reached statistical significance ( p <0.05). Conclusion The desaturases used in biosynthesis of fatty acids from their precursors may be in limited supply in certain genotypes. These mothers should insure exogenous fatty acids are being consumed. Support: Nutrition Obesity Research Center, UNC

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.032
Threshold uncertainty score0.063

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.255
Teacher spread0.243 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2012
Admission routes1
Has abstractyes

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