Mitochondrial plasticity in the anoxic turtle heart
Bibliographic record
Abstract
Our objective was to assess mitochondrial respiration and electron transport chain (ETC) activities in permiabilised cardiac fibers from normoxic and anoxic (2 weeks) turtles ( Trachemys scripta ) acclimated to 5°C. All experiments on animals were conducted in conformance with the FASEB Principles. Mitochondrial maximum ADP stimulated respiration was measured with an Oroboros micro‐respirometer, and respiratory flux through complex I, II and IV of the ETC was assessed by pharmacological intervention. ADP affinity was determined by adding ADP in steps from 0.025 mM to a maximum of 1 mM. All preparations were tested at 5, 13 and 21°C. Anoxic acclimation led to significant reductions in maximum respiration rates through ETC complexes I, II and IV at all temperatures tested, and ADP affinity was significantly lower. State II respiration rates were significantly reduced in anoxic cardiac fibers, leading to a higher respiratory control ratio, which is indicative of a reduction in proton leak. Collectively, these results suggest a reduction in mitochondrial capacity contributes to overall hypometabolism in anoxic turtles. Since complex I of the ETC is a major site of reactive oxygen species generation, a downregulation of this protein may also help to prevent redox damage during anoxic recovery. This research was funded by The Killam Memorial Trust and The Natural Sciences and Educational Research Council of Canada.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".