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E‐cadherin regulates mitochondrial metabolism and induces cell growth through NF‐κB in E‐cadherin deficient AGS cells

2017· article· en· W3177355225 on OpenAlexfundno aff
Song-Yi Park, Jee‐Hye Shin, Sun‐Ho Kee

Bibliographic record

VenueThe FASEB Journal · 2017
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMechanisms of cancer metastasis
Canadian institutionsnot available
FundersMinistère de l'Éducation, du Loisir et du Sport Québec
KeywordsGene knockdownCadherinCell growthCell biologyNF-κBWnt signaling pathwaySmall hairpin RNACateninCancer cellApoptosisChemistryBiologyCellCancer researchSignal transductionCancerBiochemistry

Abstract

fetched live from OpenAlex

In this study the interrogation of Wnt regulator β‐catenin and an emerging cancer control molecule NF‐κB was explored in E‐cadherin expressed AGS gastric cancer cells (EC96 cells). It was found that E‐cadherin can increase cell proliferation and energy metabolism through activation of NF‐κB. EC96 cells treated with NF‐κB inhibitors showed a decrease in cell survival. Knockdown of E‐cadherin expression by shRNA could clearly reverse the E‐cadherin‐induced energy metabolism potential in EC96 cells. The application of NF‐kB inhibitors supported the evidence that NF‐kB act as an upstream signal of c‐myc and Glut1. Normally NF‐kB is present in the cytosol of resting cells. Our results showed that E‐cadherin expression led NF‐κB to translocate from cytosol to nucleus in AGS cells. The treatment of NF‐kB inhibitors or knockdown with E‐cadherin shRNA abolished the ability of E‐cadherin to translocate NF‐kB into nucleus in EC96 cells, which was accompanied with the reduction of cell growth. In conclusion, E‐cadherin expression generates cell proliferation signals through NF‐κB signal pathway as Wnt signal pathway was suppressed. Support or Funding Information [This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF‐2015R1D1A1A09056775)]

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.007
Threshold uncertainty score0.750

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.252
Teacher spread0.236 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2017
Admission routes1
Has abstractyes

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