Hydrogen peroxide (H <sub>2</sub> O <sub>2</sub> ) increased the expression of Gqα in vascular smooth muscle cells: role of growth factor receptors
Bibliographic record
Abstract
We have recently shown that high glucose increased the expressions of Gqα which was attributed to the enhanced levels of H 2 O 2 . The present studies were undertaken to investigate if H 2 O 2 can mimic the effect of high glucose on the expression of Gqα and‐mediated signaling in A10 VSMC. Treatment of A10 VSMC with H 2 O 2 for 4 h increased the levels of Gqα protein in a concentration‐dependent manner. A maximal increase of ~50% in the levels of Gqα proteins was observed at 50μM of H 2 O 2 . Since H 2 O 2 transactivates growth factor receptors, we also investigated the implication of growth factor receptors in H 2 O 2 ‐mediated increase in Gqα levels by using specifics inhibitors of growth factor receptors such as AG1024 for IGF‐R or AG1295 for PDGF‐R. Pretreatment of cells with growth factor receptor inhibitors resulted in the restoration of H 2 O 2 ‐induced enhanced expression of Gqα proteins. In addition, PD‐98059, an inhibitor of mitogen‐activated protein kinase (MAPK), and wortmannin, an inhibitor of phosphatidylinositol 3‐kinase (PI3‐K), decreased H 2 O 2 –induced enhanced levels of Gqα. On the other hand, the enhanced activity of MAPK induced by H 2 O 2 was also inhibited by growth factor receptors inhibitors. These results suggest that H 2 O 2 ‐mediated up‐regulation of Gqα expression in VSMC occurs through the transactivation of growth factor receptor‐induced activation of the MAPK/PI3K pathways. (Supported by Quebec Heart Foundation)
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".