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Record W3177714474 · doi:10.21203/rs.3.rs-15889/v1

Isolation nontuberculous mycobacteria and histopathological changes in lymph nodes collected at the abattoir in cattle reactive-positive to tuberculin dermal test

2020· preprint· en· W3177714474 on OpenAlexaff
Angélica Hernández-Jarguín, Julio Martı́nez-Burnes, Gloria María Molina‐Salinas, Ned I. de la Cruz-Hernández, José L. Palomares-Rangel, Hugo Barrios-García

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldMedicine
TopicMycobacterium research and diagnosis
Canadian institutionsUniversity of Prince Edward Island
FundersUniversidad Autónoma de TamaulipasConsejo Nacional de Ciencia y Tecnología
KeywordsNontuberculous mycobacteriaIsolation (microbiology)LymphTuberculin testTuberculinPathologyMedicineVeterinary medicineTuberculosisMicrobiologyBiologyMycobacterium

Abstract

fetched live from OpenAlex

Abstract Background: The Mycobacterium tuberculosis complex causes a variety of diseases; in bovine, the common pathogen is M. bovis which is considered zoonotic. A separate group of mycobacteria, much less known, is "non-tuberculous mycobacteria (NTM) which are also infectious for animals and humans. The Mexican Official Norm (NOM-ZOO-031-1995) regulates M. bovis in cattle, but not NTM species, even though this last type of microorganisms has a confounding effect for the diagnosis of bovine tuberculosis. The objective of the study was to isolate and identify the NTM of bovine lymph nodes condemned in the slaughterhouse, to characterize the histological lesions in these tissues and to correlate bacteriological and postmortem findings with the antemortem skin test of the tuberculin. Results: Mycobacteria were isolated from 54/528 (10.2%) of the lymph nodes; 29/54 (53.7%) of these isolates were identified as M. bovis and 25/54 (46.2%) as NTM; 3 bacteriological cultures were discarded due to contamination with fungi and in one case it was not possible to identify the species. Granulomatous and pyogranulomatous inflammation were observed in 6/21 (28.6%) and 7/21 (33.3%) of the NTM-positive lymph nodes, respectively. Necrosis and mineralization were only found in 6/21(28.6%) of the lymph nodes. The species of NTM associated with granulomatous lymphadenitis were M. scrofulaceum, M. triviale, M. terrae and M. szulgai, while those causing pyogranulomatous lesions were M. szulgai, M. kansasii, M. phlei, and M. scrofulaceum. Conclusions: Considering the increase of mycobacterial infections in humans worldwide, the idetification of NTM that inducing Tuberculosis-like lesions in abattoir inspection is the first step to investigate the livestock-human-wildlife-environment interactions with especially focus on transmission dynamics.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0010.001
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.025
GPT teacher head0.289
Teacher spread0.263 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations1
Published2020
Admission routes1
Has abstractyes

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