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Record W3177942710 · doi:10.1073/pnas.2105195118

Structural basis for potassium transport in prokaryotes by KdpFABC

2021· article· en· W3177942710 on OpenAlexfundno aff
Marie Sweet, Casper K. Larsen, Xihui Zhang, Michael Schlame, Bjørn Panyella Pedersen, David L. Stokes

Bibliographic record

VenueProceedings of the National Academy of Sciences · 2021
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicIon Transport and Channel Regulation
Canadian institutionsnot available
FundersOffice of ScienceNational Institutes of HealthAarhus Universitets ForskningsfondBiological and Environmental ResearchEuropean CommissionNational Institute of General Medical SciencesAarhus UniversitetSimons FoundationYork UniversityU.S. Department of Energy
KeywordsCytoplasmProtein subunitBiophysicsChemistryIon transporterATPaseATP hydrolysisStereochemistryTransporterSUPERFAMILYStructural biologyBiochemistryMembraneEnzymeBiologyReceptorGene

Abstract

fetched live from OpenAlex

Significance Potassium is the major intracellular cation in all kingdoms of life. In bacteria, potassium is used to maintain membrane potential, pH, and to provide turgor pressure for cell growth and division. When external potassium levels are low, an ATP-dependent pump called KdpFABC is essential to maintain intracellular potassium levels. This protein complex comprises a subunit descending from potassium channels and another from P-type ATPases. We used cryogenic electron microscopy to investigate this unique partnership and to address mechanisms of energy coupling and transport. We present structures corresponding to all major reaction intermediates and map distinct densities along the proposed transport pathway. Based on this data, we describe a mechanism that is unique amongst known membrane transporters.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.101
Threshold uncertainty score0.162

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.275
Teacher spread0.254 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations35
Published2021
Admission routes1
Has abstractyes

Explore more

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