S-palmitoylation of NOD2 controls its localization to the plasma membrane
Bibliographic record
Abstract
Nucleotide-binding and oligomerization domain containing protein 2 (NOD2) is a cytosolic pattern-recognition receptor that detects intracellular peptidoglycan (muramyl dipeptide) from bacteria. Membrane association of NOD2 is essential for its ability to activate nuclear factor κB and mitogen-activated protein kinase signaling pathways via the kinase, receptor-interacting serine/threonine-protein kinase 2. The post-translational addition of palmitate to NOD2 results in an acylated protein with increased affinity for membrane bilayers (1Lu Y. Zheng Y. Coyaud É. Zhang C. Selvabaskaran A. Yu Y. Xu Z. Weng X. Chen J.S. Meng Y. Warner N. Cheng X. Liu Y. Yao B. Hu H. et al.Palmitoylation of NOD1 and NOD2 is required for bacterial sensing.Science. 2019; 366: 7Crossref Scopus (60) Google Scholar). Palmitoylation of cysteine residues (shown in orange) at positions C395 and C1033 en face of the structural model (A) based on the crystal structure of rabbit NOD2 (PDB ID: 5IRL) (2Maekawa S. Ohto U. Shibata T. Miyake K. Shimizu T. Crystal structure of NOD2 and its implications in human disease.Nat. Commun. 2016; 7: 11813Crossref PubMed Scopus (102) Google Scholar) is mediated by the protein acyltransferase enzyme zDHHC5 (1Lu Y. Zheng Y. Coyaud É. Zhang C. Selvabaskaran A. Yu Y. Xu Z. Weng X. Chen J.S. Meng Y. Warner N. Cheng X. Liu Y. Yao B. Hu H. et al.Palmitoylation of NOD1 and NOD2 is required for bacterial sensing.Science. 2019; 366: 7Crossref Scopus (60) Google Scholar). The model suggests that this face of the protein is juxtaposed to the plasmalemmal surface. Palmitoylation of the expressed GFP-NOD2WT and GFP-NOD2C395,1033S in HCT116 cells was further characterized using a biorthogonal chemical reporter assay (B) (3Yap M.C. Kostiuk M.A. Martin D.D.O. Perinpanayagam M.A. Hak P.G. Siddam A. Majjigapu J.R. Rajaiah G. Keller B.O. Prescher J.A. Wu P. Bertozzi C.R. Falck J.R. Berthiaume L.G. Rapid and selective detection of fatty acylated proteins using ω-alkynyl-fatty acids and click chemistry.J. Lipid Res. 2010; 51: 1566-1580Abstract Full Text Full Text PDF PubMed Scopus (89) Google Scholar). Here, 15-hexadecynoic acid (15-HDYA), also referred to as alkynyl palmitic acid, was metabolically incorporated into cells. The 15-HDYA covalently attached to the immunocaptured GFP-NOD2 proteins was reacted with azide-PEG3-FLAG via a copper-catalyzed azide–alkyne cycloaddition reaction. The proteins were resolved by SDS-PAGE and subsequently detected by an anti-FLAG antibody. The metabolic label, which was incorporated into NOD2WT and was notably absent in the double cysteine mutant, was removed by treatment with 2.5% hydroxylamine that hydrolyzes the 15-DYA–protein thioester linkage. Finally, the palmitoylation-deficient GFP-NOD2C395,1033S does not localize to the plasma membrane in HCT116 cells (C). Mutations that disrupt S-palmitoylation are associated with severe immunologic and inflammatory diseases such as Crohn’s disease, whereas mutations associated with Blau syndrome demonstrate increased S-palmitoylation and membrane localization (1Lu Y. Zheng Y. Coyaud É. Zhang C. Selvabaskaran A. Yu Y. Xu Z. Weng X. Chen J.S. Meng Y. Warner N. Cheng X. Liu Y. Yao B. Hu H. et al.Palmitoylation of NOD1 and NOD2 is required for bacterial sensing.Science. 2019; 366: 7Crossref Scopus (60) Google Scholar). EQUIPMENT: The following equipment were used: A Zeiss Axiovert 200M microscope with a Hamamatsu ImageEM x2 camera, Yokogawa spinning disc, and a 63× 1.4 NA oil objective. REAGENTS: Alkynyl-palmitate (Click Chemistry Tools), azide-PEG3-FLAG (Jena Bioscience), hydroxylamine, and other copper-catalyzed azide–alkyne cycloaddition reagents (TCEP, CuSO4, and TBTA) were obtained from MilliporeSigma. SOFTWARE: The following software were used: MetaMorph, ImageJ, Python, PyMOL, and AutoDock (4Trott O. Olson A.J. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading.J. Comput. Chem. 2009; 31: 455-461Google Scholar). This work is supported by the Canadian Institutes of Health Research Project Grants PJT166010 awarded to GDF.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".