Co-Production of Isobutanol and Ethanol from Prairie Grain Starch Using Engineered Saccharomyces cerevisiae
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Isobutanol is an important and valuable platform chemical and an appealing biofuel that is compatible with contemporary combustion engines and existing fuel distribution infrastructure. The present study aimed to compare the potential of triticale, wheat and barley starch as feedstock for isobutanol production using an engineered strain of Saccharomyces cerevisiae. A simultaneous saccharification and fermentation (SSF) approach showed that all three starches were viable feedstock for co-production of isobutanol and ethanol and could produce titres similar to that produced using purified sugar as feedstock. A fed-batch process using triticale starch yielded 0.006 g isobutanol and 0.28 g ethanol/g starch. Additionally, it is demonstrated that Fusarium graminearum infected grain starch contaminated with mycotoxin can be used as an effective feedstock for isobutanol and ethanol co-production. These findings demonstrate the potential for triticale as a purpose grown energy crop and show that mycotoxin-contaminated grain starch can be used as feedstock for isobutanol biosynthesis, thus adding value to a grain that would otherwise be of limited use.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it