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Record W3194019333 · doi:10.1038/s41467-021-24932-w

ZNF768 links oncogenic RAS to cellular senescence

2021· article· en· W3194019333 on OpenAlexafffund
Romain Villot, Audrey Poirier, Inan Bakan, Karine Boulay, Erlinda Fernández, Romain Devillers, Luciano Gama-Braga, Laura Tribouillard, Andréanne Gagné, Éma Duchesne, Danielle Caron, Jean-Sébastien Bérubé, Jean‐Christophe Bérubé, Yan Coulombe, Michèle Orain, Yves Gélinas, Stéphane Gobeil, Yohan Bossé, Jean‐Yves Masson, Sabine Elowe, Steve Bilodeau, Venkata Manem, Philippe Joubert, Frédérick A. Mallette, Mathieu Laplante

Bibliographic record

VenueNature Communications · 2021
Typearticle
Languageen
FieldMedicine
TopicCancer-related Molecular Pathways
Canadian institutionsCentre hospitalier de l'Université LavalHôtel-Dieu de QuébecCentre hospitalier universitaire de QuébecUniversité LavalUniversité de MontréalHôpital Maisonneuve-RosemontInstitut universitaire de cardiologie et de pneumologie de Québec
FundersCIHR Skin Research Training CentreFonds de Recherche du Québec - SantéNatural Sciences and Engineering Research Council of CanadaInstitut universitaire de cardiologie et de pneumologie de Québec, Université LavalGovernment of CanadaCanadian Cancer Society Research InstituteCanadian Institutes of Health ResearchCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of CanadaUniversité Laval
KeywordsSenescenceCell biologyCell growthBiologyCarcinogenesisCell cycleSignal transductionPhosphorylationOncogeneEffectorCellCancerGenetics

Abstract

fetched live from OpenAlex

RAS proteins are GTPases that lie upstream of a signaling network impacting cell fate determination. How cells integrate RAS activity to balance proliferation and cellular senescence is still incompletely characterized. Here, we identify ZNF768 as a phosphoprotein destabilized upon RAS activation. We report that ZNF768 depletion impairs proliferation and induces senescence by modulating the expression of key cell cycle effectors and established p53 targets. ZNF768 levels decrease in response to replicative-, stress- and oncogene-induced senescence. Interestingly, ZNF768 overexpression contributes to bypass RAS-induced senescence by repressing the p53 pathway. Furthermore, we show that ZNF768 interacts with and represses p53 phosphorylation and activity. Cancer genomics and immunohistochemical analyses reveal that ZNF768 is often amplified and/or overexpressed in tumors, suggesting that cells could use ZNF768 to bypass senescence, sustain proliferation and promote malignant transformation. Thus, we identify ZNF768 as a protein linking oncogenic signaling to the control of cell fate decision and proliferation.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesResearch integrity
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.798
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.021
GPT teacher head0.313
Teacher spread0.292 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations26
Published2021
Admission routes2
Has abstractyes

Explore more

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