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Record W3201789062

Enzymatic Hydrolysis of Flaxseed to Produce Alpha-Linoleic and Linolenic Acid

2020· article· en· W3201789062 on OpenAlex
Dylan Scheibelhoffer

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

aboutThe title or abstract carries a Canadian signal from the geographic lexicon.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueArchives in Chemical Research · 2020
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEnzyme Catalysis and Immobilization
Canadian institutionsnot available
Fundersnot available
KeywordsLipaseFood scienceChemistryLinoleic acidHydrolysisFatty acidLinolenic acidBiochemistryEnzyme
DOInot available

Abstract

fetched live from OpenAlex

Microbial lipases have numerous potential applications in the bioprocessing industry due to their variety and versatility. Canada, in particular Saskatchewan is one of the worldwide leaders in the production of flaxseed crops accounting for 40% of crop production. Flaxseed oil is a viable source of plant based α-linoleic and linolenic acids (omega-6 and omega-3). Flaxseed oil contains high amounts of linolenic acid and moderate amounts of linoleic acid. The concentration of these essential free fatty acids in flaxseed oil can be increased via the process of enzymatic hydrolysis. Alpha-linoleic and linolenic acid are high value nutritional supplements in great demand to the pharmaceutical and health industries. Humans cannot synthesize these fatty acids within the body and must consume them in their diets or in the form of supplements thus, increasing the desired free fatty acid content within flaxseed oil is a viable solution to this need. The focus of the present work is to produce α- linoleic and linolenic free fatty acids catalyzed by microbial lipases. In addition, the optimization of the enzymatic hydrolysis reaction conditions and use of flax seed oil as feedstock and growth medium will be studied in the present work. Initial experiments showed an increase of 78 wt. % of free fatty acid yield following optimized hydrolysis using lipase from Aspergillus niger and 96% wt. % increase using lipase from Candia rugosa.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.367

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.041
GPT teacher head0.336
Teacher spread0.295 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it