Development of Polymorphic SSR Markers in <i>Vallisneria</i> Based on RAD seq
Bibliographic record
Abstract
Vallisneria belongs to Hydrocharitaceae, which is a pioneer species of ecological restoration in waters. In order to carry out a more comprehensive SSR analysis of Vallisneria , we used the restriction-site associated DNA sequencing (RAD-seq) technology to analyze Simple Sequence Repeats (SSR) information of Vallisneria , SSR markers and primer of Vallisneria were developed based on RAD-seq data．Among which ,366 simple sequence repeats (SSR) loci were detected. Primers of 355 loci were designed successfully, and the two base repeat type SSR loci were the largest (59.56%). Finally, we confirmed 23 pairs of polymorphic SSR primers after the screening and validation. The analysis results by Genepop software showed that the mean number of alleles of the 23 loci is 3.26, and these loci do not link to each other (P<0.01). Four loci deviate from HWE (P<0.01) and the number of heterozygous roughly the same as that of homozygotes (observed heterozygosity mean of 0.460), inbreeding coefficient is high (mean of 0.880). Which could be caused by take sample too closely in the same sampling, and Vallisneria have a combination of generative propagation and clone.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.002 | 0.002 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.005 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".