O-225 Exploring the impact of night shift work and melatonin on methylation in circadian genes
Bibliographic record
Abstract
Introduction Night shift work is associated with increased cancer risk, but the molecular mechanisms are not well-understood. It is hypothesized that melatonin suppression due to night shift work could impact DNA methylation in circadian genes, although this has been evaluated by few studies. Objectives This study explored the relationship between night shift work parameters and patterns of melatonin secretion on methylation in circadian genes among women. Methods A cross-sectional study was conducted in 2019–2020 among 74 female healthcare employees who participated in a previous study in which urinary melatonin levels were evaluated over a 48-hour period. Participants provided information on demographics, lifestyle behaviors, and night shift work such as current night shift work pattern, duration in years, and intensity (consecutive nights). The Illumina Infinium MethylationEPIC beadchip was applied to DNA extracted from new blood samples to measure methylation at 1150 CpG loci across 22 circadian genes. Multiple linear regression was used to examine the association between night shift work, melatonin parameters and methylation levels at each CpG site, while accounting for the false-discovery rate (q=0.2). Results Compared to day workers, night shift workers had hypermethylation in the promoter region of CSNK1E (q=0.15). Women who worked night shifts for ≥10 years exhibited hypomethylation in the body of NR1D1 (q=0.08) compared to those with < 1 0 years of history. Hypermethylation in the body of ARNTL was also apparent for those who worked ≥3 consecutive night shifts a week compared to < 3 nights (q=0.18). Among night shift workers, melatonin patterns (24-hr concentrations, peak timing) were associated with methylation at three loci (RORA, MTNR1A, PER3) (q≤ 0.20). No association between melatonin and methylation was identified among day workers. Conclusion These findings suggest that circadian misalignment among night shift workers is associated with differential methylation in several circadian genes, but larger studies are needed to confirm.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.006 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".