Evaluarea eficacităţii testului real-time PCR în detectarea la naştere a streptococului de grup B comparativ cu cultura clasică
Bibliographic record
Abstract
Introduction. Group B Streptococcus (GBS) – or Streptococcus agalactiae (a Gram-positive bacterium) – is responsible for some neonatal conditions or even neonatal death. Data from some studies and some European recommendations call into question the intrapartum screening, due to the existence of false positive/negative cases at the screening from 35-37 weeks of gestation. In this situation, it is recommended to use in the labor ward a point-of-care system based on real-time molecular PCR technology, which allows to obtain fast and high accuracy results. Materials and method. In order to evaluate the effectiveness of the real-time PCR test in the intrapartum detection of the vaginal colonization of group B Streptococcus, we conducted a prospective study by evaluating 100 intrapartum pregnant women hospitalized in the Nova Vita Hospital Maternity, using the GBS DS kit and the Revogene system produced by GenePOC Canada, whose results we compared with those obtained through traditional culture. Results. At the end of the study, 83 pregnant women were eligible for evaluation. The incidence of vaginal group B Streptococcus at 35-37 weeks detected by traditional culture was 14.63%. The intrapartum incidence of GBS presence detected by traditional culture was 7.31% (six cases), and by real-time PCR (Revogene system), it was 9.75% (eight cases), of which four new cases detected intrapartum (4.87%). The sensitivity of the PCR test at birth was 83.33%, the specificity was 96.05% (95% CI; 88.9-99.2) and AUC was 89.7% (95% CI; 81-95.3). Conclusions. Point-of-care systems, such as Revogene system, can be easily used in the labor ward, the results being available in a short period (70 minutes), and real-time PCR molecular technology (NAAT) can detect more patients colonized with group B Streptococcus intrapartum than classical culture and, thus, the medical staff can prevent the complications of group B Streptococcus infection in the newborn and mother.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.004 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.000 | 0.003 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.006 | 0.004 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; both teacher heads agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".