Cloning and Expression Analysis of <i>EdAGPL1</i> in <i>Eleocharis dulcis</i>
Bibliographic record
Abstract
In order to clone the cDNA sequence of water chestnut AGPase large subunit gene ( EdAGPL1 ) and analyze its structural characteristics and its expression in different tissues and corm development of water chestnut. Using water chestnut 'Guilin water chestnut ' as research material. The EdAGPL1 gene was cloned by RT-PCR and analyzed by bioinformatics. The expression of EdAGPL1 in different tissues and corm development process was analyzed by real-time fluorescence quantitative PCR technology. The results showed that the length of the cloned EdAGPL1 gene was 1 744 bp, its open reading frame was 1 599 bp, it encoded 532 amino acids, the protein molecular mass was 58.84 kD, the isoelectric point was 7.54, and it had NTP_transferase and PbH1 domains. The secondary structure was composed of helix (13.72%), for the strand (25.19%) and the loop (61.09%). The tertiary structure consists of 17 α-helices, 34 β-sheets, and 51 β-turn (curl) composition. Homology and phylogenetic analysis showed that the homology of amino acid sequence of EdAGPL1 and other plants AGPL protein was 57.54%~61.64%, and has far evolutionary kinship with other plants. Fluorescence quantitative PCR analysis showed that the expression of EdAGPL1 in different tissues was corm> leaf stem > stolon > root. The expression of EdAGPL1 was the highest in the initial stage of corm development, and then it decreased and remainedstable. The study of this gene will help to clarify the regulation mechanism of starch synthesis in water chestnut and provide a theoretical basis for the breeding of high-starch varieties.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".