Telomerase Activity of Apple Plantlets <i>in Vitro</i> Kept Relative Stable after Conserved for Years
Bibliographic record
Abstract
In order to understand the telomerase characteristics in relation to the proliferation and aging of the tissue culture plantlets of apple, the leaves, stem segments and callus sampled from Malus domestica ‘Golden Delicious’, Malus domestica ‘Fuji’ and Malus domestica ‘Gala’ plantlets that subcultured in vitro for years were analyzed for the telomerase activity and the amount of telomerase reverse transcriptase gene expression by using modified TRAP and Quantitative Real-time PCR. The results showed that the telomerase activity in the leaves and stem segments had no significant difference among the plantlets that subcultured for different times. During a given subculture time, the telomerase activity in leaves showed a tendency of increasing first and then declining along with the leaf aging in a ninety-day period. The telomerase activity varied in different materials, among which Malus domestica ‘Golden Delicious’ callus and Malus domestica ‘Gala’ leaves appeared the highest amount, respectively. The expression of telomerase reverse transcriptase gene was consistent with the change of telomerase activity.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".