A Comprehensive Pedagogy for Dialectic Team-based Marketing Management Case Analysis.
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Electrosprayed protein ions can retain native-like conformations. The intramolecular contacts that stabilize these compact gas-phase structures remain poorly understood. Recent work has uncovered abundant salt bridges in electrosprayed proteins. Salt bridges are zwitterionic BH<sup>+</sup>/A<sup>-</sup> contacts. The low dielectric constant in the vacuum strengthens electrostatic interactions, suggesting that salt bridges could be a key contributor to the retention of compact protein structures. A problem with this assertion is that H<sup>+</sup> are mobile, such that H<sup>+</sup> transfer can convert salt bridges into neutral B<sup>0</sup>/HA<sup>0</sup> contacts. This possible salt bridge annihilation puts into question the role of zwitterionic motifs in the gas phase, and it calls for a detailed analysis of BH<sup>+</sup>/A<sup>-</sup> versus B<sup>0</sup>/HA<sup>0</sup> interactions. Here, we investigate this issue using molecular dynamics (MD) simulations and electrospray experiments. MD data for short model peptides revealed that salt bridges with static H<sup>+</sup> have dissociation energies around 700 kJ mol<sup>-1</sup>. The corresponding B<sup>0</sup>/HA<sup>0</sup> contacts are 1 order of magnitude weaker. When considering the effects of mobile H<sup>+</sup>, BH<sup>+</sup>/A<sup>-</sup> bond energies were found to be between these two extremes, confirming that H<sup>+</sup> migration can significantly weaken salt bridges. Next, we examined the protein ubiquitin under collision-induced unfolding (CIU) conditions. CIU simulations were conducted using three different MD models: (i) Positive-only runs with static H<sup>+</sup> did not allow for salt bridge formation and produced highly expanded CIU structures. (ii) Zwitterionic runs with static H<sup>+</sup> resulted in abundant salt bridges, culminating in much more compact CIU structures. (iii) Mobile H<sup>+</sup> simulations allowed for the dynamic formation/annihilation of salt bridges, generating CIU structures intermediate between scenarios (i) and (ii). Our results uncover that mobile H<sup>+</sup> limit the stabilizing effects of salt bridges in the gas phase. Failure to consider the effects of mobile H<sup>+</sup> in MD simulations will result in unrealistic outcomes under CIU conditions.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it