The small intestinal lactase expression is reduced in the piglet with bowel inflammation
Bibliographic record
Abstract
The effects of dextran sodium sulfate (DSS) induced bowel inflammation on jejunal lactase activity and expression were examined in 12 Yorkshire piglets at 5 d of age with body weight (BW) of 3 kg were fitted with ig catheters and divided into control (CON) and treatment groups (DSS). Both groups of pigs were infused with an equal volume of either saline or 1.25 g DSS/kg BW.d in saline, respectively, for 10 d. Lactase protein abundance was carried out by Western blotting and the mRNA abundance was measured by quantitative real time RT‐PCR. â‐actin was used as the housekeeping gene. DSS treatment decreased (P<0.05) jejunal lactase maximal specific activity (Vmax, µmol/mg protein.min) in the tissue homogenate (CON, 87.66±0.14 vs. DSS, 24.05±0.30) by 73%, cytosol (CON, 45.83±0.68 vs. DSS, 12.58±1.15) by 73% and apical membrane (CON, 422.67±3.29 vs. DSS, 92.22±2.42) by 78%, respectively, with orresponding decreases (P<0.05) of 26, 48, and 32% in the jejunal lactase protein abundance. The relative abundance of the jejunal lactase mRNA was lower by 25% in the DSS piglets. Bowel inflammation reduced the lactase activity in the small intestine by lowering its expression at the transcriptional and translational levels. Supported by AFMNet, OMAFRA and NSERC of Canada.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".