Anoxia/reoxygenation‐induced cardiac myocyte apoptosis: role of HMGB1
Bibliographic record
Abstract
It has been proposed that ischemia/reperfusion (I/R)‐induced myocardial dysfunction is due to myocytes apoptosis. However, the mechanism(s) involved in myocyte apoptosis after I/R is not completely clear, but appears to involve cytokines. High mobility group box 1 (HMGB1) is a nuclear non‐histone DNA binding protein that has been implicated in cell apoptosis. The aim of the present study was to assess the role of HMGB1 in myocyte apoptosis‐induced by anoxia/reoxygenation (A/R, in vitro counter part of I/R). Cultured murine cardiac myocytes were challenged with a 30 min of anoxia followed by 48 hrs of reoxygenation. The A/R challenge increased myocyte apoptosis (Caspase 3 activity and Cell Death ELISA). Concomitantly, there was an increase in HMGB1 expression in cardiac myocytes. Further, the A/R‐induced increase in myocyte apoptosis was prevented when the myocytes were pretreated with a HMGB1 inhibitor (glycyrrhizic acid). Finally, exposure of the cardiac myocytes to medium containing HMGB1 (1 μg/ml) for 24 hrs resulted in myocyte apoptosis. Taken together, our findings indicate that HMGB1 production by cardiac myocytes plays an important role in A/R‐induced myocyte apoptosis. (CIHR MOP 81303).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".