Correction to ‘The double-stranded RNA-binding protein, Staufen1, is an IRES-transacting factor regulating HIV-1 cap-independent translation initiation’
Bibliographic record
Abstract
Errors were introduced in Figure 3 during production of the article (1). The X-axis of Figure 3D should be ΔSV40 not SV40. The publisher apologises for these errors and wishes to correct Figure 3 as shown below. Staufen1 does not enhance alternative splicing of the dl HIV-1 IRES RNA nor increases the cryptic promoter activity of the dl HIV-1 DNA. (A, B) The dl HIV-1 IRES (150 ng) was cotransfected with a control scRNA (100 nM) or with siRLuc (100 nM), in the presence, or the absence (−), of the Stau155-HA3 (325 ng) plasmid. (A) Schematic representation of the dl reporter targeted by the siRNA RLuc (siRLuc) targeting the Renilla luciferase ORF (upper panel). Total protein extracts were prepared 48 hrs post-transfection. The expression of Stau155-HA3 was determined by western blot, using the GAPDHprotein as a loading control (lower panel). (B) RLuc and FLuc activities were measured and expressed relative to the values obtained with scRNA, set to 100% (RLA). Values shown are the mean (±SEM) for six independent experiments, each performed in duplicate. Statistical analysis was performed by an ordinary two-way ANOVA test (*P < 0.01; ns, not significant). (C, D) HEK 293T cells were transfected with either the dl HIV-1 IRES (150 ng) or a promoterless ΔSV40-dl HIV-1 IRES (150 ng) vector in the presence, or the absence (−), of the Stau155-HA3 (325 ng) plasmid. 24 hrs post-transfection total protein extracts were prepared. (C) Schematic representation of the dl HIV-1 IRES and ΔSV40-dl HIV-1 IRES plasmids (upper panel). The expression of Stau155-HA3 was determined by western blot, using the GAPDH protein as a loading control (lower panel). (D) RLuc and FLuc activities were measured, and results are expressed as RLA relative to the activities obtained from the dl HIV-1 IRES vector when in the absence of the Stau155-HA3, set to 100%. Values shown in are the mean (±SEM) for three independent experiments, each performed in duplicate. Statistical analysis was performed by an ordinary two-way ANOVA test (**** P < 0.0001; ns, not significant). Staufen1 does not enhance alternative splicing of the dl HIV-1 IRES RNA nor increases the cryptic promoter activity of the dl HIV-1 DNA. (A, B) The dl HIV-1 IRES (150 ng) was cotransfected with a control scRNA (100 nM) or with siRLuc (100 nM), in the presence, or the absence (−), of the Stau155-HA3 (325 ng) plasmid. (A) Schematic representation of the dl reporter targeted by the siRNA RLuc (siRLuc) targeting the Renilla luciferase ORF (upper panel). Total protein extracts were prepared 48 hrs post-transfection. The expression of Stau155-HA3 was determined by western blot, using the GAPDHprotein as a loading control (lower panel). (B) RLuc and FLuc activities were measured and expressed relative to the values obtained with scRNA, set to 100% (RLA). Values shown are the mean (±SEM) for six independent experiments, each performed in duplicate. Statistical analysis was performed by an ordinary two-way ANOVA test (*P < 0.01; ns, not significant). (C, D) HEK 293T cells were transfected with either the dl HIV-1 IRES (150 ng) or a promoterless ΔSV40-dl HIV-1 IRES (150 ng) vector in the presence, or the absence (−), of the Stau155-HA3 (325 ng) plasmid. 24 hrs post-transfection total protein extracts were prepared. (C) Schematic representation of the dl HIV-1 IRES and ΔSV40-dl HIV-1 IRES plasmids (upper panel). The expression of Stau155-HA3 was determined by western blot, using the GAPDH protein as a loading control (lower panel). (D) RLuc and FLuc activities were measured, and results are expressed as RLA relative to the activities obtained from the dl HIV-1 IRES vector when in the absence of the Stau155-HA3, set to 100%. Values shown in are the mean (±SEM) for three independent experiments, each performed in duplicate. Statistical analysis was performed by an ordinary two-way ANOVA test (**** P < 0.0001; ns, not significant). The published article has been updated. These corrections do not affect the results, discussion and conclusions presented in the article.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.026 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.002 |
| Bibliometrics | 0.003 | 0.002 |
| Science and technology studies | 0.002 | 0.002 |
| Scholarly communication | 0.002 | 0.002 |
| Open science | 0.004 | 0.002 |
| Research integrity | 0.003 | 0.009 |
| Insufficient payload (model declined to judge) | 0.064 | 0.049 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".