1014. Effect of Levofloxaclin and Trimethoprim-sulfamethoxazole on Planktonic and Biofilm Cells of <i>Stenotrophomonas maltophilia</i> Isolates Obtained from Blood and Respiratory Samples
Bibliographic record
Abstract
Abstract Background Stenotrophomonas maltophilia is a non-fermenting Gram-negative pathogen, which causes healthcare-associated infections and exhibits increasing drug resistance rates. In this study, we compared the effect of levofloxacin and trimethoprim-sulfamethoxazole on planktonic and biofilm cells of S. maltophilia isolates obtained from blood and respiratory samples. Methods S. maltophilia isolates were recollected from patients older than 18 years from 2018-2020 from Hospital Universitario Dr. Jose Eleuterio Gonzalez, a tertiary care hospital from Nuevo Leon, Mexico. Bacteria were dentified by MALDI-TOF MS. Biofilm formation was determined using the crystal violet staining method.Antimicrobial susceptibility in planktonic cells and biofilm cells were determined by the broth microdilution and the Calgary device, respectively. Results During the 3-year study period, 44 S. maltophilia isolates were identified, 28.8% of which were biofilm producers. Of those, 69.2% were obtained from blood cultures. Isolates were obtained from several wards: 25% infectious diseases ward, 18% nephrology, 18% intensive care units, 14% geriatric ward, 7% plastic surgery, and 18% from other wards. The majority of the studied subjects were men (34.6%), and the mean age was 41 years. The majority of the isolates were susceptible to levofloxacin (94.1%) and to trimethoprim-sulfamethoxazole (76.4%) in planktonic assays. All the isolates were resistant to both antibiotics when analyzing biofilm cells. Levofloxacin resistance was at least four-fold higher in biofilm cells compared to planktonic cells. Trimethoprim-sulfamethoxazole resistance was at least ten-fold higher in biofilm cells compared to planktonic cells. Conclusion The majority of S. maltophilia clinical isolates causing blood infections in a hospital in Mexico were able to produce biofilm. The production of biofilm in S. maltophilia strains enhanced resistance to levofloxacin and trimethoprim-sulfamethoxazole. Disclosures All Authors: No reported disclosures
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".