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Abstract CC04-01: First-in-human biomarker-driven phase I TRESR trial of ataxia telangiectasia and Rad3-related inhibitor (ATRi) RP-3500 in patients (pts) with advanced solid tumors harboring synthetic lethal (SL) genomic alterations

2021· article· en· W4200468047 on OpenAlexaff
Timothy A. Yap, Elizabeth Lee, David R. Spigel, Elisa Fontana, Martin Højgaard, Stéphanie Lheureux, Niharika B. Mettu, Louise Carter, Ruth Plummer, Danielle Ulanet, Peter Manley, Ying Jiang, Ezra Y. Rosen

Bibliographic record

VenueMolecular Cancer Therapeutics · 2021
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCRISPR and Genetic Engineering
Canadian institutionsPrincess Margaret Cancer Centre
Fundersnot available
KeywordsZygosityGermlineMedicineInternal medicineNeutropeniaBiomarkerCancer researchOncologyBiologyGeneticsChemotherapyGene

Abstract

fetched live from OpenAlex

Abstract Background: RP-3500 is a novel potent and selective ATRi. A genome-wide CRISPR/Cas9-based screening platform (SNIPRx) was utilized to identify and validate synthetic lethal (SL) genomic alterations that predict sensitivity to RP-3500. This is the first presentation of data from the ongoing Phase I TRESR (Treatment Enabled by SNIPRx) study. METHODS: Pts with advanced solid tumors harboring RP-3500 sensitizing alterations were recruited. Pts received oral RP-3500 in 21-day cycles on different doses and schedules using a BOIN design. Pharmacokinetic (PK) and pharmacodynamic (PD) studies were conducted in serial tumor and/or blood samples. Biomarker tests included somatic and germline NGS, including zygosity studies, ATM and γH2AX IHC, and circulating tumor DNA (ctDNA). RESULTS: 62 pts (mean age 62 y, 42% males, 43% ≥5 prior lines of therapy) received RP-3500 (range: 1–8+ cycles). 44 pts remained on RP-3500 at the June 4, 2021, data cut-off. Tumors with ATM (n=26), BRCA1/2 (n=16), CDK12 (n=5) and other (n=20) molecular alterations were included. Germline (22 germline and 15 somatic, 25 pending) and zygosity status (10 bi-allelic and 5 mono-allelic, 46 pending) will be presented. Treatment-related adverse events (TRAEs) were mostly Grade (G)1 and transient. TRAEs occurring in >10% of pts were limited to anemia (all grades 37%, G3 26%, G4–5 0%) and fatigue (all grades 13%, G3 2%). Other ≥G3 TRAEs occurred in <5% of pts and included thrombocytopenia (4.8%) and neutropenia (1.6%). No pts discontinued RP-3500 due to TRAEs, 17/62 discontinued due to progressive disease or clinical progression and 1/62 due to withdrawal of consent. RP-3500 plasma exposures showed a dose-dependent increase with T1/2 of 6 hrs. Once-daily dosing was sufficient to meet RP-3500 exposure requirements set from pre-clinical studies. Tumor γH2AX induction (median increase =140%; p-value =0.02) was seen across doses and genotypes confirming target modulation. Declines in variant allele frequencies (>50%) in ctDNA were observed in 8/14 evaluable pts and correlated with antitumor activity (Pearson correlation coefficient =0.65; p-value =0.015). Of 31 pts evaluable for response, 14 (45%) had tumor regression on at least 1 radiologic evaluation. Objective responses were seen in 6 pts: 4 RECISTv1.1 partial responses in tumors with CDK12 and BRCA1 alterations (2 confirmed; 2 unconfirmed), and 2 PCWG3 in tumors with ATM loss. Six pts had stable disease ≥4 cycles. CONCLUSIONS: The TRESR study represents the largest biomarker-driven FIH trial for a single agent ATRi and validates prospective pt selection based on the presence of SL genomic alterations. RP-3500 shows a robust PK/PD profile, preliminary anti-tumor activity, and predictable and manageable on-target anemia (<30% G3, no G4/5) with low potential for off-target toxicity. Enrollment continues, including combinations with PARPi and other therapies. Clinical trial information: NCT04497116. Citation Format: Timothy Yap, Elizabeth Lee, David Spigel, Elisa Fontana, Martin Højgaard, Stephanie Lheureux, Niharika B. Mettu, Louise Carter, Ruth Plummer, Danielle Ulanet, Peter Manley, Ying Jiang, Ezra Rosen. First-in-human biomarker-driven phase I TRESR trial of ataxia telangiectasia and Rad3-related inhibitor (ATRi) RP-3500 in patients (pts) with advanced solid tumors harboring synthetic lethal (SL) genomic alterations [abstract]. In: Proceedings of the AACR-NCI-EORTC Virtual International Conference on Molecular Targets and Cancer Therapeutics; 2021 Oct 7-10. Philadelphia (PA): AACR; Mol Cancer Ther 2021;20(12 Suppl):Abstract nr CC04-01.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.044
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.303
Teacher spread0.292 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations4
Published2021
Admission routes1
Has abstractyes

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