BsmI AND HinfI POLYMORPHISMS OF THE FABP4 GENE OF DIFFERENT PIG BREEDS OF THE BELGOROD REGION IN RUSSIA
Bibliographic record
Abstract
PIGS were genotyped for FABP3 BsmI (G5005A) and HinfI (C406T) gene. The study examined 188 boars of four breeds (Duroc, Large White, Landrace and Yorkshire) from the Belgorod region of Russia. Fatty acid binding proteins (FABP) are involved in the regulation of metabolism, bind and transport fatty acids, and play an important role in the regulation of fat accumulation and distribution.Purpose of the study: to assess the frequencies of alleles and genotypes for BsmI and HinfI polymorphisms in FABP4 gene among the Duroc, Large White, Landrace and Yorkshire breeds at the farms of the Belgorod region of Russia. Materials and methods: Genomic DNA was isolated from alcoholized earmarks. Pigs were genotyped by PCR-RFLP method. The PCR product in the amount of 10 l was hydrolyzed with 4 units of the corresponding restriction enzyme BsmI or HinfI (SibEnzyme, Russia) for 16 hours. The hydrolysis products were separated by horizontal electrophoresis in 2% agarose gel (Mini-Sub Cell GT, BioRad, USA). Gel blocks were stained with ethidium bromide (0.5 g/ml) and visualized on a UV transilluminator to detect DNA fragments. Result: For C406T mutation, the presence of the T mutant allele was noted only for the Duroc breed (0.467), and the C allele was fixed for the other breeds. According to the G5005A mutation, the highest frequency of the A allele associated with the content of intramuscular fat increase was noted among Duroc pigs (0.440), and the smallest -in the Yorkshire breed (0.213). According to the 2 test, all studied breeds, except for monomorphic groups, are in the state of genetic equilibrium according to Hardy-Weinberg. Conclusion: Our data on the variability of these markers generally corresponds to previously published materials for the pigs of Chinese, Canadian and Korean selection. At the same time, the increase of the A BsmI allele frequencies is associated with a high content of IMF for the pigs of the Belgorod region, which may be associated with the result of breeding work to improve the meat quality.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".