Molecular characterization of Spodoptera litura Nucleopolyhedrovirus (SlNPV) Labuhan Batu isolate
Bibliographic record
Abstract
Abstract Spodoptera litura F. is a new pest that potentially becomes a serious pest threatening palm oil industry. The control can be carried out using biological control agents S.litura Nucleopolyhedrovirus ( Sl NPV). Molecular approach using polymerase chain reaction (PCR) has been used to characterize the Sl NPV isolate. The aim of this study was to determine the molecular characteristics of Sl NPV Labuhan Batu isolate based on DNA sequence analysis. The NPV isolate was collected from oil palm plantation Negeri Lama Estate Group, PT. Hari sawit Jaya, Asian Agri, Labuhan Batu. The Sl NPV DNA extraction was conducted using the DNA gSYNC kit. The amplification of Sl NPV DNA was done by specific primers with oligonucleotide sequences forward 5′-ACGTTACGATCTAGATCC-3′ and reverse 5′-AACGTTAGACAGACATAT-3′ with ±700bp of amplicon. Homology and phylogeny characteristics were used to determine the relationship of Sl NPV isolate from Labuhan Batu to NPV from other countries were reported in the GeneBank of National Center for Biotechnology Information (NCBI). The results showed that Sl NPV isolate from Labuhan Batu had the closest related to Sl NPV from Australia, Sli NPV from Canada and Sl NPV from China, with the similarity percentage 100%, 96% and 90% respectively. They belong to the same group as the NPVs that infected the Spodoptera genus.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".