Combined effect of Drypetes gossweileri essential oil and organic acids on the inhibition of Bacillus spore germination using response surface methodology
Bibliographic record
Abstract
Abstract This work assessed the combined effect of Drypetes gossweileri essential oil (EO) and three organic acids (Na + benzoate, Na + lactate and Sorbic acid) on the inhibition of germination and growth of Bacillus spores. The test consisted of inoculating activated spores of each Bacillus species in 4 ml of nutrient broth under the combined effect of D. gossweileri EO (0.62, 5.31 and 10 µg/ml), each organic acid (0.01, 0.05 and 0.1 %) at different pH levels (4.5, 5.75 and 7.0) taken as independent variable. After incubating for 48 hours, the inhibition percentages of spores germination were used to generate the polynomial model: Y (%) = β 0 - β 1 X 1 + β 2 X 2 + β 3 X 3 + β 1,1 X 1 2 + β 1,2 X 1 X 2 + β 1,3 X 1 X 3 + β 2,2 X 2 2 + β 2,3 X 2 X 3 + β 2,3 X 3 2 . The ANOVA test was used to determine how significant (p ˂ 0.05) of each factor, their interactions and the validity of the final models. The results showed that the determination coefficient (R 2 ) and the adjusted coefficients (R 2 adj) were generally close. For each bacterial species, all the models were significant (p ˂ 0.05). For each organic acids, the interaction effect with D. gossweileri EO (X 1 X 2 ) was positive and significant (p ˂ 0.05) in at least one bacterial species out of the four tested. Na + lactate showed a more positive and significant (p ˂ 0.05) interactions with EO, as observed on B. megaterium, B. subtilis and G. stearothermophilus spores with regression coefficients of 23.54; 15.83 and 7.41 respectively. Decreasing the pH increases the sensitivity of the spores to D. gossweileri EO and organic acids. Indeed at pH 7.0, no total inhibition of spore germination was obtained while at 5.75, total inhibition of spore germination (100% inhibition) was observed for B. cereus and G. stearothermophilus for Na + benzoate ; G. stearothermophilus for Na + lactate and B. cereus , B. megaterium and B. subtilis for Sorbic acid. From these results, it was concluded that D. gossweileri EO combined with organic acid might be a valuable tool for bacterial spores control in the food industries.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.006 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.001 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".