Impact of broiler breeder growth trajectory on plasma corticosterone concentration: a comparison of analytical methods
Bibliographic record
Abstract
Blood concentration of corticosterone (CORT) is a measure of welfare in feed restricted broiler breeders. The RIA and ELISA have been routinely used for measuring CORT in blood, excreta, and feather. Due to the presence of some confounding factors in the aforementioned colorimetric enzyme reaction methods, some methodological difficulties have been attributed to those assays. The correlation between broiler breeder plasma CORT concentrations, measured using ELISA and a novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, was the focus of the current study. A total of 36 broiler breeder pullets were used, of which 30 were randomly assigned to one of 10 unique growth trajectories, and 6 were assigned to an unrestricted group. We designed the growth trajectories using a 3-phase Gompertz growth model with 10 levels of BW gain in the prepubertal and pubertal growth phases, ranging from the breeder-recommended target BW (CON) to 22.5% higher (CON+22.5%), in 2.5% increments. The BW trajectories were applied to each individual bird using a precision feeding (PF) system, which collected BW and feed intake data. The birds were classified based on age at first egg (AFE), and 12 birds each having the highest and lowest AFE was selected for the CORT study. Then median photostimulation BW of the candidate birds was used to define the upper (heavy BW) and lower (standard BW) extremes, and plasma CORT levels were evaluated by ELISA and LC-MS/MS methods from their blood collected at 18, 20, 22, 24, and 26 wk of age. Concentrations of plasma CORT measured using ELISA method were highly correlated (r = 0.95; P < 0.001) with values measured using LC-MS/MS method, validating interchangeably usage of both methods to measure plasma CORT in broiler breeders. Plasma CORT levels were not affected by photostimulation BW or breeders' age, indicating same welfare status between the precision fed high and low BW groups.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".