Cage transplant experiment shows weak transport effect on relative abundance of fish community composition as revealed by eDNA metabarcoding
Why this work is in the frame
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Bibliographic record
Abstract
Protection of freshwater fish diversity is a global conservation priority in face of its alarming decline in the last decades. A crucial step to protect freshwater fish diversity is the production of prompt and precise evaluation of community composition and spatial distribution. Metabarcoding of environmental DNA (eDNA metabarcoding) generally surpasses traditional methods for documenting diversity and community composition in aquatic environments. Nevertheless, empirical evidence evaluating how eDNA transportation in water affect community composition and structure via eDNA metabarcoding data remains scarce. Using a brown trout (Salmo trutta) cage transplant experiment in the St. Lawrence River (Canada), a large fluvial system, we tested the detection and relative abundance of species’ eDNA along 15 sampling locations. We detected brown trout eDNA in five localities up to 5,000 m from the cage, but only one sampling location situated 10 m downstream and in the direct line of the cage was affected at the community composition level. This locality showed a relative abundance of brown trout eDNA of 13.1%, while the four others showed a relative abundance under 1.0%. K-means cluster analysis confirmed the impact of brown trout eDNA on community composition by separating this locality from all others. Based on species loading of a redundancy analysis, we showed that this different k-means group was associated with the high relative abundance of brown trout. No evidence of transport effect of brown trout eDNA on fish community composition was observed in any other sampling locations. Together, our results support the view that eDNA metabarcoding can be both a conveyor belt of biodiversity information and a precise tool to study the composition and structure of fish communities in river.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.006 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it