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Record W4220847491 · doi:10.1080/27694127.2022.2047267

snz/SNX25 at the crossroad of endocytosis and lipid handling in autophagy

2022· article· en· W4220847491 on OpenAlexafffund
Annie Lauzier, Steve Jean

Bibliographic record

VenueAutophagy Reports · 2022
Typearticle
Languageen
FieldMedicine
TopicAutophagy in Disease and Therapy
Canadian institutionsUniversité de Sherbrooke
FundersCanadian Institutes of Health Research
KeywordsAutophagySorting nexinCell biologyEndocytosisEndosomeBiologyBAG3RegulatorIntracellularCellGeneGeneticsApoptosis

Abstract

fetched live from OpenAlex

Macroautophagy/autophagy is coupled to a myriad of intracellular processes, among which vesicular trafficking is an important contributor of membranes and proteins required at various stages, from autophagosome formation to degradation. Hence, understanding how membrane trafficking is coupled to autophagy induction and how cells rewire trafficking upon high autophagic needs is instrumental to our understanding of autophagy. In our recent manuscript, we tested a known class of endosomal sorting regulators, the sorting nexin family, for their involvement in autophagy in Drosophila. We identified snz (snazarus) as an important regulator of autophagy in Drosophila as well as in mammalian cells, by demonstrating a role for the snz human ortholog SNX25 in HeLa cells. Using knockout rescue experiments, we observed that SNX25 loss affects many cellular processes, namely VAMP8 endocytosis and lipid handling. Mutational studies identified separate protein domains involved in these processes. Given the role of this sorting nexin family in lipid droplet regulation, our work expands their requirement to autophagy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.065
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.263
Teacher spread0.254 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes2
Has abstractyes

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