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Record W4221051037 · doi:10.5539/jas.v14n4p51

Molecular Detection of Age-Related Abundance and Diversity of Bifidobacterium spp. in Pastured Goats

2022· article· en· W4221051037 on OpenAlexvenueno aff
Eunice Ndegwa, Ako Agyemang

Bibliographic record

VenueJournal of Agricultural Science · 2022
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGut microbiota and health
Canadian institutionsnot available
FundersNational Institute of Food and AgricultureU.S. Department of Agriculture
KeywordsBifidobacteriumBiologyBifidobacterium animalisFecesVeterinary medicineLactobacillusMicrobiologyBacteriaMedicineGenetics

Abstract

fetched live from OpenAlex

Bifidobacterium spp. are among bacteria being developed as probiotics for farm animals. Understanding diversity of the normal gut inhabitants is a prerequisite to developing species-specific probiotics strains that are likely to establish in the host. For many farm animals including goats, the normal gut Bifidobacterium inhabitants have not been characterized including age based differences. The objective of this exploratory study was to gain preliminary understanding of abundance and diversity of Bifidobacterium species in goats of different ages. Molecular methods have given scientists fast methods for exploratory insights into previously uncharacterized microbiomes. Consequently, in this study we utilized molecular assays using genus and species specific primers to characterize Bifidobacterium in pastured goats. Although Bifidobacterium were detected in all ages, younger animals had higher counts than older goats. In goats below six months of age, B. angulatum, B. dentium, B. gallicum, B. animalis sub animalis, B. longum and B. catenulatum were detected. In goats six months and older, B. dentium and B. gallicum were predominant. In some goats however, the specific strains could not be identified with the currently available primers indicating there are goat specific unique strains that are yet to be characterized. Further research to characterize and isolate Bifidobacterium in goats are needed for future probiotic applications. In conclusion, Bifidobacterium spp. were common in all age groups of pastured goats but more abundant in pre-weaned goats compared to goats over six months. In addition age related differences in the diversity of Bifidobacterium spp. in goats were reported.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.211
Teacher spread0.206 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2022
Admission routes1
Has abstractyes

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