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Subcellular Protein Expression of Annexin A1 (ANXA1) in Different Types of Cancer

2022· article· en· W4225419539 on OpenAlexaff
Karyn Olascuaga‐Castillo, Susana Rubio‐Guevara, Elena Cáceres‐Andonaire, Olga E. Caballero-Aquiño, Elena Mantilla‐Rodríguez, Julio Hilario‐Vargas, José Andrés Morgado‐Díaz, Maxim V. Berezovski

Bibliographic record

VenueThe FASEB Journal · 2022
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicS100 Proteins and Annexins
Canadian institutionsUniversity of Ottawa
FundersUniversidad Nacional de Trujillo
KeywordsSubcellular localizationCytoplasmAnnexin A1StainingNuclear localization sequenceBiologyCancerPathologyCell biologyAnnexinMedicineGenetics

Abstract

fetched live from OpenAlex

Annexin A1 (ANXA1) is a Ca2+‐regulated phospholipid‐binding protein involved in several physiological functions. Further research has shown that this protein might have various roles in cancer development and operate at different levels. This is partially related to the placement of ANXA1 in distinct cellular compartments. ANXA1 is maybe nuclear, cytoplasmic, and membrane‐associated. The goal of this study has been to analyze ANXA1 subcellular localization and expression in different types of cancer. Methods We examined 1295 data sets available in The Human Protein Atlas (HPA) (HPA). The research examined the ANXA1 expression in tissue from 1130 cancer and 165 normal samples. A visual categorization method based on staining intensity (I), the proportion of stained cells (F) (F). ANXA1 expression levels as a function of subcellular localization. Q Score (product of E*I) was calculated by the fraction of stained cells (F) and the intensity (I) (I) Results In cancer patients, the predominant subcellular localization was cytoplasmic membranous (53.3 %), whereas, in healthy individuals, the principal was cytoplasmic membranous and nuclear (57.0 %). In terms of staining intensity (Q Score), the highest values were found at the cytoplasmic membranous and nuclear levels (Q Score=5.69). In contrast, the highest Q Score values were found in standard samples at the nuclear level (Q Score=6.0). Conclusion The subcellular localization of ANXA1 expression may be a helpful diagnostic marker in various cancer types.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.005

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0020.002
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.243
Teacher spread0.232 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2022
Admission routes1
Has abstractyes

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