GENERATION OF TRANSGENIC WHEAT PLANTS RESISTANT TO SALINITY
Bibliographic record
Abstract
Почвенное засоление – один из основных процессов деградации сельскохозяйственных земель. В суровых климатических условиях произрастания многих продовольственных культур необходимо внедрять сорта нового поколения, сорта, созданные методами биотехнологии. Такие сорта уже возделываются в таких биотехнологических странах, как Австралия, США, Канада и с недавнего времени в Аргентине. В настоящее время доказано, что генетические механизмы солеустойчивости определяются десятками генов и факторами транскрипции, работа которых важна для реакций растений на солевой стресс. В своей работе мы использовали ген транскрипционного фактора osgata риса, эктопическая экспрессия которого инициируется избыточным засолением и дефицитом воды. Мы показали, что ген транскрипционного фактора osgata в трансгенной пшенице обеспечивает улучшенную устойчивость к соленому стрессу и показатели роста. Трансгенные растения пшеницы получали путем биобаллистической генетической трансформации конструкцией, несущей полноразмерную копию гена osgata под злакоспецифичным Act1 промотором и селективный ген bar. ПЦР-анализ трансгенных растений Т0 и Т1 подтвердил интеграцию osgata в геном пшеницы. Стабильную экспрессию osgata в геноме пшеницы дополнительно подтверждали полуколичественным анализом ОТ-ПЦР. По сравнению с нетрансформированными растениями, трансгенные растения, экспрессирующие osgata, продемонстрировали лучшие показатели роста и улучшенную защиту от повреждения клеток при солевом стрессе. Soil salinization is one of the main processes of agricultural land degradation. In the harsh climatic conditions of the growth of many food crops, it is necessary to introduce new generation varieties, varieties created by biotechnology methods. Such varieties are already being cultivated in biotech countries such as Australia, the USA, Canada, and more recently in Argentina. It has now been proven that the genetic mechanisms of salt tolerance are determined by dozens of genes and transcription factors, essential for plant responses to salt stress. In our work, we used the rice osgata transcription factor gene, the ectopic expression initiated by excessive salinity and water deficit. We have shown that the osgata transcription factor gene in transgenic wheat provides improved salt stress tolerance and growth performance. Transgenic wheat plants were obtained by bioballistic genetic transformation with a construct carrying a full-length copy of the osgata gene under the cereal-specific Act1 promoter and a selective bar gene. PCR analysis of transgenic T0 and T1 plants confirmed the integration of osgata into the wheat genome. Stable osgata expression in the wheat genome was additionally confirmed by semi-quantitative RT-PCR analysis. Transgenic plants expressing osgata showed better growth performance and improved protection against cell damage under salt stress compared to nontransformed plants.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".