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Record W4236124366 · doi:10.7287/peerj.preprints.2071

Proline content and varying barometric pressure: an analysis of six genes, across three marine a<i>ltererythrobacter s</i>trains

2016· preprint· en· W4236124366 on OpenAlexaff
Conor Barrie

Bibliographic record

Venuenot available
Typepreprint
Languageen
FieldEnvironmental Science
TopicMicrobial Community Ecology and Physiology
Canadian institutionsUniversity of British Columbia, Okanagan CampusUniversity of British Columbia
Fundersnot available
KeywordsBayProlineTaxonomy (biology)BiologyStrain (injury)BotanyEcologyOceanographyAmino acidGeologyGenetics

Abstract

fetched live from OpenAlex

Environmental stresses select for suitably adapted genes. These stresses will promote the propagation of certain mutations, and the loss of others, throughout the evolution of a species. For organisms living two thousand meters below the ocean’s surface, one of these stresses is certainly high barometric pressure. Due to the imino ring structure of proline it was hypothesized that bacteria sampled from greater depths would show a larger proportion of content in their proteins. This hypothesis was examined by analyzing the proline content of 6 proteins common to 3 species from the altereythrobacter speciesl All the selected strains are Gram negative, moderately halophilic marine bacteria with optimum temperatures around 30⁰C. A. marensis strain KCTC22370 (taxonomy ID: 543877) was sampled, and genomically annotated, by Soo and Lee from the surface of the sea near Jeju island in the Republic of North Korea (2010). A. epoxidivorans strain JCS350 (taxonomy ID: 361183) was sampled from cold-seep sediment in the Kagoshima Bay, off the coast of Japan, at an approximate depth of 120m. The strain was isolated and genomically annotated by Kwon et.al. (2007). A. atlanticus strain 26DY36 (taxonomy ID: 1267766) was sampled from a North Atlantic mid ocean range at a depth of 2577m. The strain was isolated and annotated by Wu et. al. (2015). The data showed no significant differences in the proline content of the 3 species examined, however a more expansive study would be required to fully reject the hypothesis.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.002
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.044
GPT teacher head0.276
Teacher spread0.232 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2016
Admission routes1
Has abstractyes

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