PUBLICATION
Bibliographic record
Abstract
Objective: Diffuse large B cell lymphoma (DLBCL) incidence rates have increased year by year, a part of them have poor clinical outcomes, but the underlying mechanism involved is still unclear.Chemokines have been thought to play an important role in occurrence and development of tumors, but they are poorly studied in DLBCL.CC-Chemokine Ligand 2 (CCL2), the most representative of the CC chemokine family members, through binding to its high affinity receptor, CC chemokine receptor 2 (CCR2), has be regarded to involve in tumor growth, angiogenesis, epithelial mesenchymal transition, metastasis and immune escape etc. in recent years, but the role and mechanism in DLBCL has not been reported yet.Our preliminary study showed high expression of CCL2 or CCR2 was correlated with clinicopathological characteristics, and an adverse prognostic factor for overall survival (OS) and progression-free survival (PFS) of DLBCL patients.The purpose of this study is to investigate the role of CCL2-CCR2 axis signaling in DLBCL by in vitro experiment.Methods: CCL2 and CCR2 expression were analyzed in human DLBCL cell lines and normal B lymphocytes by Western blot (WB) and qPCR.CCL2 and CCR2 genes were silenced by lentivirus infection.The proliferation, migration, apoptosis and signaling pathway were detected by CCK8, transwell, flow cytometry (FC) and WB, respectively.Results: CCL2 and CCR2 were expressed in all human DLBCL cell lines (SUDHL-2, SUDHL-4, SUDHL-6, OCI-Ly8 and OCI-Ly10).Blockade of CCL2-CCR2 axis signaling with lentivirus infection, CCL2 neutralizing antibody or CCR2 antagonist inhibited tumor cell proliferation, migration and anti-apoptosis ability.The CCL2-CCR2 axis involved in the proliferation and migration of DLBCL cells by activating PI3K/Akt signaling pathway, and induced apoptosis through activation of P38MARK signaling pathway.Conclusions: Our study demonstrates that CCL2/CCR2 axis signaling plays an important role in the development of DLBCL by stimulating cell proliferation, migration and anti-apoptosis.The inhibition of CCL2 or CCR2 may, therefore, be a potential target for anticancer therapy in DLBCL.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.010 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.003 | 0.002 |
| Science and technology studies | 0.002 | 0.001 |
| Scholarly communication | 0.007 | 0.003 |
| Open science | 0.001 | 0.002 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.473 | 0.307 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; the direct Gemma label and the distilled Codex classifier agree on what is shown here.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".