Trafficking of green fluorescent protein tagged‐vesicular acetylcholine transporter to varicosities in a cholinergic cell line
Bibliographic record
Abstract
The publishers wish to apologize for the substandard printing of Figs 4 and 5 of the above article that appeared in J. Neurochem.78, pp. 1104–1113. We reproduce Figs 4 and 5 below as they should have appeared. GFP–VAChT is present in early and recycling endosomes. SN56 cells were transfected with GFP–VAChT and examined by confocal microscopy (a and c). (b) Labeling of cells in (a) with Tfn-TxR for 5 min at 37°C. Arrows indicate some of the structures labeled by both GFP–VAChT and Tfn-TxR. Bar, 10 μm. (d) Labeling of cells in (c) with Tfn-TxR for 20 min at 37°C. Arrows indicate colocalization of GFP–VAChT and transferrin in recycling endosomes. The arrowhead shows colocalization in early endosomes. Bar, 20 μm. GFP–VAChT is present in Rab5-positive endosomes. (a) Cells were transfected with GFP–Rab5 construct, and living cells were examined by serial Z-sectioning. The maximum Z projection is presented. (b) Image of the same cell as in (a) stained with FM4-64 for 30 min. Arrows indicate colocalization of GFP–Rab5 and FM4-64. (c) Coexpression of GFP–VAChT and the constitutively activated Rab5 mutant Q79L. Cells were examined by serial Z-sectioning and the maximum Z projection is shown. Expression of Rab5:Q79L results in larger endosomes visualized after incubation with FM4-64 (c2). GFP–VAChT (c1) also appears in structures larger than that observed normally (e.g. Figs 1, 2, 3 and 4), which have FM4-64 (c2). Bars, 20 μm.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".