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Record W4244013818 · doi:10.1002/0470028637.met042

Calpain

2004· other· en· W4244013818 on OpenAlexaff
Peter L. Davies, Robert L. Campbell, Tudor Moldoveanu

Bibliographic record

VenueHandbook of Metalloproteins · 2004
Typeother
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCalpain Protease Function and Regulation
Canadian institutionsQueen's University
Fundersnot available
KeywordsCalpainProteasesProteasePapainActive siteGene isoformChemistryEnzymeCysteine proteaseBiochemistryCatalytic triadIntracellularBinding siteCell biologyBiophysicsStereochemistryBiologyGene

Abstract

fetched live from OpenAlex

Abstract Calpains are intracellular cysteine proteases that participate in Ca 2+ signaling. Some structural information is available for the ubiquitous mammalian isoforms m‐ and μ‐calpain. These 110‐kDa heterodimeric, multidomain enzymes differ in the level of Ca 2+ required for activation. A structural explanation for the Ca 2+ requirement is that the papain‐like active site is not assembled in the Ca 2+ ‐free (apo) state. Moreover, the circular arrangement of domains appears to constrain the two protease core domains from assembling into an active conformation. Ca 2+ binding to the two penta–EF‐hand domains and to a C2‐like domain flanking the protease core may release these constraints. However, active site assembly requires the cooperative binding of Ca 2+ to two non–EF‐hand sites, one on each of the catalytic domains. These novel Ca 2+ binding sites appear to be an evolutionary addition to the papain‐like protease core. Thus, Ca 2+ acts at several different levels, at different sites, to tightly control the activity of this potent protease within the cell.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Other · Consensus signal: Other
Teacher disagreement score0.504
Threshold uncertainty score0.999

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.215
Teacher spread0.209 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designNot applicable
Domainnot available
GenreOther

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2004
Admission routes1
Has abstractyes

Explore more

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