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New three-stage in vitro model for infant colonic fermentation with immobilized fecal microbiota

2006· article· en· W4244297653 on OpenAlexaff
Cécile Cinquin, Gwenaëlle Le Blay, Ismaı̈l Fliss, Christophe Lacroix

Bibliographic record

VenueFEMS Microbiology Ecology · 2006
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicProbiotics and Fermented Foods
Canadian institutionsUniversité Laval
Fundersnot available
KeywordsBiologyFecesFermentationMicrobiologyIn vitroFood scienceBiochemistry

Abstract

fetched live from OpenAlex

In the paper by Cinquin et al. (2006), some footnote symbols were reproduced incorrectly in Tables 2 and 3, and there was also an error in the symbols in the legend to Fig. 2. Bacterial populations measured by plate counts and fluorescence in situ hybridization in effluent samples from the three reactor stages at the pseudo-steady state and in fecal inocula for fermentation F1 (13 and 10 g L−1 of carbohydrate concentrations) and F2 (10 g L−1 of carbohydrate concentrations) FISH counts significantly different from plate counts at the significance level: *P<0.05, **P<0.005, ***P<0.0005. Effects of fermentation section (PCS, TCS and DCS) on bacterial populations: values with different letters (capitals for plate counts and small letters for FISH) are significantly different by Tukey-Kramer HSD test (P<0.05). Data are mean values calculated for the last 4 and 2 days for each fermentation period for plate counts and FISH, respectively. PCS, proximal colon simulation; TCS, transverse colon simulation; DCS, distal colon simulation; PC, plate counts; nd, not determined; ND, not detected, value below the detection threshold of the FISH method which was 6.8 Log 10 cell number per mL for effluent samples and 7.5 Log 10 cell number per g for wet feces. Metabolite concentrations and ratios in effluent samples from the three reactor stages at the pseudo-steady state and fecal inocula for fermentation F1 (13 and 10 g L−1 of carbohydrates) and F2 (10 g L−1 of carbohydrates) SCFA concentrations in F1 (10 g L−1 total carbohydrate) significantly different from SCFA concentrations in F1 (13 g L−1 total carbohydrate) at the significance level: *P<0.05, **P<0.005, ***P<0.0005. Effects of fermentation section (PCS, TCS and DCS) on metabolites concentrations: values with different letters are significantly different by Tukey-Kramer HSD test (P<0.05). ‡Pooled SEM, pooled standard errors of the mean. Data are mean values calculated for the last 4 days of each period. PCS, proximal colon simulation; TCS, transverse colon simulation; DCS, distal colon simulation; PC, plate counts; nd, not determined; ND, not detected, value below the detection threshold of the HPLC method (2 mM). Viable bacterial counts of total and facultative anaerobe populations in proximal (PCS) and distal (DCS) effluent from fermentation F2 with total carbohydrate concentration of 10 g L−1: total anaerobes (), bifidobacteria (), bacteroides (), clostridia (), facultative anaerobes (), Gram+cocci (), staphylococci (), coliforms (), lactobacilli (), pH (—). RBC (repeated batch cultures) corresponding to beads precolonization. Tables 2 and 3 and Fig. 2 are reproduced correctly here. The publishers apologize for this error.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.012

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0020.002
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.001
Open science0.0020.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.214
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2006
Admission routes1
Has abstractyes

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