Affinity interactions of Actin with HS1 and Annexin I, II, IV, V and VI in RAW264.7 macrophages in vitro and in the Fc-receptor complex
Bibliographic record
Abstract
Actin binding proteins HS1 and Annexin I, II, III, IV, V and VI proteins were examined in RAW macrophages in vitro using affinity chromatography, LC-MS/MS, western blot, cell staining and expression of fluorescent proteins. Actins and many related actin isoforms were observed in the phagosome. However, actins were also observed on polystyrene beads incubated with crude extracts readily bound to NHS affinity chromatography beads in the absence of anti-actin antibodies. Annexin II and V and the haematopoietic specific proteins HS1 (also called Lyn substrate) were prominently observed in isolated phagosomes and not observed in control beads incubated with crude extracts of RAW macrophages. Annexin II and V as well as G3BP, FLT1, FARP2, STARD13, RAB25, and FRAP1 were observed to bind actin in affinity chromatography experiments using LC-MS/MS and western blot. Cell staining and/or expression of GFP constructs showed that actin, HS1 and Annexin V prominently accumulate at the nascent Fc-receptor complex of RAW macrophages.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".