Disulfide bond formation of heterodimer and heterotrimer of human laminin-332 coiled-coil domains
Bibliographic record
Abstract
Laminin (LM) α, β, and γ chains were connected by disulfide bonds at the C-and N-termini of the LM coiled-coil (LCC) domain to form heterodimers and heterotrimers.At the Cterminus of LCC domain, one disulfide bond is formed to connect β and γ chains while it was unclear how disulfide bond pattern is formed to connect α, β, and γ chains at the Nterminus of LCC domain.Using an insect cell-free translation system, we succeeded to produce heterotrimers of LCC domain of human LM-332.To analyze disulfide bond formation at the N-terminus of LCC domain, we mutated cysteines of LCC domains into alanines by site-directed mutagenesis and co-expressed these mutants in an insect cell-free translation system.Mutation of a single cysteine at the N-terminus of LCC domain of one chain caused the failure of disulfide bond formation of heterotrimers.However, mutation of cysteines at the N-terminus of LCC domain of two different chains recovered the disulfide bond formation of heterotrimers with different efficiencies.These results suggest that the disulfide bond patterns at the N-terminus of human LM-332 LCC domains are not specific.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".