Peer Review #2 of "Effects of the loss of estrogen on the heart’s hypertrophic response to chronic left ventricle volume overload in rats (v0.1)"
Bibliographic record
Abstract
Aortic valve regurgitation (AR) can result in heart failure from chronic overloading of the left ventricle (LV).Little is known of the role of estrogens in the LV responses to this condition.The aim of the study was to compare LV remodeling in female rats with severe AR in absence of estrogens by ovariectomy (Ovx).In a first study, we investigated over 6 months the development of hypertrophy in 4 groups of female Wistar rats: AR or shamoperated (sham) and Ovx or not.Ovx reduced normal heart growth.As expected, volume overload (VO) from AR resulted in significant LV dilation (42% vs. 32% increase LV enddiastolic diameter in intact and Ovx groups vs. their respective sham group; p<0.0001).LV weight was also significantly and similarly increased in both AR groups (non-Ovx and Ovx).Increase in stroke volume or cardiac output and loss of systolic function were similar between AR intact and AR Ovx groups compared to sham.We then investigated what were the effects of 17beta-estradiol (E2; 0.03 mg/kg/day) treatment on the parameters studied in Ovx rats.Ovx reduced uterus weight by 85% and E2 treatment restored up to 65% of the normal weight.E2 also helped normalize heart size to normal values.On the other hand, it did not influence the extent of the hypertrophic response to AR.In fact, E2 treatment further reduced LV hypertrophy in AR Ovx rats (41% over Sham Ovx + E2).Systolic and diastolic functions parameters in AR Ovx + E2 were similar to intact AR animals.Ovx in sham rats had a significant effect on the LV gene expression of several hypertrophy markers.Atrial natriuretic peptide (Nppa) gene expression was reduced by Ovx in sham-operated females whereas brain natriuretic peptide (Nppb) expression was increased.Alpha (Myh6) and beta (Myh7) myosin heavy chain genes were also significantly modulated by Ovx in sham females.In AR rats, LV expression of both Nppa and Nppb genes were increased as expected.Ovx further increased it of AR rats for Nppa and did the opposite for Nppb.Interestingly, AR in Ovx rats had only minimal effects on Myh6 and Myh7 genes whereas they were modulated as expected for intact AR animals.In summary, loss of estrogens by Ovx in AR rats was not accompanied by a worsening of hypertrophy or PeerJ reviewing
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.006 | 0.040 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.004 | 0.002 |
| Science and technology studies | 0.004 | 0.001 |
| Scholarly communication | 0.005 | 0.002 |
| Open science | 0.002 | 0.003 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.259 | 0.150 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".