Immunodeficiency Canada Satellite Symposium at 2014 Canadian Society of Allergy and Clinical Immunology Annual Scientific MeetingOctober 23, 2014Delta Ottawa CentreOttawa, ON
Bibliographic record
Abstract
Chronic Granulomatous Disease (CGD) is a primary immunodeficiency caused by mutations that disable the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme complex.This dysfunction subsequently leads to decreased or absent production of reactive oxygen species required for phagocytic killing of certain pathogens.CGD can be inherited in an X-linked or autosomal recessive fashion.The different genetic forms of CGD vary in their residual NADPH oxidase function and can thus be differentiated based on this quantification.Importantly, residual reactive oxygen species production has been correlated with long-term prognosis for patients with CGD (Kuhns et al. 2010).Dihydrorhodamine 123 is a non-fluorescent probe that oxidizes in the presence of reactive oxygen species to fluorescent Rhodamine 123.The Dihydrorhodamine 123 assay uses flow cytometry techniques to quantify the level of fluorescence in activated neutrophils which reflects the levels of reactive oxygen species.This assay allows both the diagnosis of patients with CGD and the differentiation between X-linked and autosomal recessive forms of the disease.This assay can also be used to identify X-linked carriers and predict which specific molecular defect a patient might carry.Using a case of X-linked CGD, this presentation highlights the diagnostic and prognostic utility of the Dihydrorhodamine 123 assay.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.003 | 0.001 |
| Scholarly communication | 0.003 | 0.001 |
| Open science | 0.001 | 0.002 |
| Research integrity | 0.002 | 0.002 |
| Insufficient payload (model declined to judge) | 0.375 | 0.068 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".