The astrocyte-enriched gene <i>CG11000</i> plays a crucial role in the development, locomotion and lifespan of <i>D. melanogaster</i>
Bibliographic record
Abstract
Abstract The brain of Drosophila melanogaster is a complex organ with various cell types, orchestrating the physiology and behaviors of the fly. While each cell type in the Drosophila brain is known to express a unique set of genes, their complete genetic profile is still unknown. Advances in the RNA-sequencing techniques at single cell resolution facilitate identifying novel cell type-specific markers and/or examining the specificity of the available markers. In this study, exploiting a single cell RNA sequencing data of Drosophila optic lobe (which comprises two thirds of the brain with extensive cell type diversity), we first categorized cell types based on their known molecular markers, then the genes with enriched expression in astrocytes were identified. Consistent with previous findings, the known glial markers CG34335 , Inx2 and nrv2 as well as the astrocytic genes CG9394 , Eaat1 , Gat , Gs2 and CG1552 exhibited enriched expression in the identified astrocyte cluster. Moreover, we identified CG11000 as a gene with positive expression correlation with the astrocytic marker Eaat1 . The positive expression correlation between CG11000 and Eaat1 genes was also observed in the single-cell RNA-sequencing data of Drosophila mid-brain as well as in the bulk RNA-sequencing data of Drosophila whole brain during development. Immunostaining of the brains dissected from adult flies showed overlapping fluorescence signals of CG11000 and Eaat1 expression, supporting co-expression of these genes in a set of single cells in Drosophila optic lobe. At the physiological level, RNAi-mediated suppression of CG11000 impeded th normal development of male flies without any effects on females. In adult flies, CG11000 suppression affected the locomotion activity and lifespan of D. melanogaster in an astrocyte-specific manner, suggesting pivotal role of CG11000 gene in astrocytes.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.002 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".