Examining the role of the promoter region of het-6 in escape from heterokaryon incompatibility in Neurospora crassa
Bibliographic record
Abstract
Heterokaryon incompatibility (HI) is a form of nonself recognition that limits the formation of heterokaryons -cells that contain genetically dissimilar nuclei.HI is controlled by several het loci.One of these, het-6 is a supergene complex that encodes two tightly-linked incompatibility genes, un-24 and het-6, with allelic variants Oak Ridge (OR) and Panama (PA), and only two natural haplotypes -un-24 OR het-6 OR or un-24 PA het-6 PA .het-6 encodes a HET domain protein with no known function outside of incompatibility, whereas un-24 encodes incompatibility function and the large subunit of ribonucleotide reductase.Strains incompatible at the het-6 locus can 'escape' from HI by mutation in the het-6 gene (Type I, ~97%), or suppress HI by mutation in a transcription factor called vib-1 (Type II, ~3%).This study examined the possible role of the het-6 promoter region in the initiation of escape, by introducing an ectopic het-6 OR allele into a strain that carried a null endogenous het-6 OR allele (IB20).Self-incompatible transformants exhibited delayed escape and aberrant post-escape behavior.A vib-1 mutant allele was introduced into IB20, and the resulting strain was also transformed with the ectopic het-6 OR .Transformants displayed Type-II like phenotype, but had slower growth rates than a typical Type II strain.Finally, we constructed a plasmid containing the het-6 OR allele in which the putative VIB-1 + binding site was deleted.Transformation of IB20 with this construct generated a transformant with a Type II-like growth phenotype.This study provides insight into the process of escape.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".