Synergistic effect of C2H2 type Zinc-finger protein with LEA promoter to enhance abiotic stress tolerance in 'Brassica juncea'
Bibliographic record
Abstract
The Cys2/His2 (C2H2) type zinc finger (ZF), and Late-embryogenesis abundant (LEA) proteins are associated with various cellular processes that play an important role in plant development and abiotic stress tolerance. The study was designed to evaluate the role of PLEA1:BcZF1 to enhance abiotic stress tolerance in Brassica juncea. The Group 4 LEA, LEA4-1, and ZF proteins isolated from B. napus and B. carinata respectively; were expressed in B. juncea cv. varuna. Expression of ZF protein in B. juncea under the control of LEA promoter showed increased tolerance against multiple abiotic stresses: salt, oxidative and drought. The increased level in the stability of total cellular membrane was observed in the transgenic lines (ZL1, ZL2 and ZL4) of B. juncea. The phenotypic analysis of transgenic lines also showed increased level of root and shoot length as compared to wild type (WT) plants under abiotic stresses. Our study suggest that cDNA encoding BcZF1 and the promoter LEA1 function as regulatory molecules involved in stabilizing and modulating the optimal plant growth under various abiotic stresses.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".